| Final A | Team | time | -time | L | React. | 50m |
| Final B | Team | time | -time | L | React. | 50m | | 9. Ariel Weech | --- | 57.58s | | 5 | 0.69s | 1. | 27.32s | | 10.Caroline Lapierre | --- | 57.70s | 0.12s | 4 | | 2. | 27.34s | | 11.Wendy Rodriguez | --- | 58.03s | 0.45s | 3 | | 6. | 28.07s | | 12.Karen Torrez | --- | 58.84s | 1.26s | 6 | 0.70s | 4. | 27.97s | | 13.Chinyere Pigot | --- | 59.03s | 1.45s | 2 | | 3. | 27.94s | | 14.Allyson Ponson | --- | 59.16s | 1.58s | 7 | 0.81s | 5. | 28.06s | | 15.Karen Nathalia Riveros | --- | 59.96s | 2.38s | 1 | | 7. | 28.88s | | 16.Julimar Avila | --- | 1:00.51 | 2.93s | 8 | 0.80s | 8. | 29.27s |
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| World record: | | Pan American Games record: |
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| Qualification: | | Standard A: 57.6s | | Standard B: 59.3s |
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| Heats | Team | time | -time | L | React. | 50m | Heat | | 1. Amanda Kendall | --- | 54.82s | | 4 | | 1. | 26.50s | Heat 1. | | 2. Vanessa Garcia | --- | 55.73s | 0.91s | 4 | 0.73s | 1. | 26.57s | Heat 2. | | 3. Erika Acuff Erndl | --- | 55.98s | 1.16s | 5 | | 2. | 27.13s | Heat 3. | | 4. Jennifer Beckberger | --- | 56.15s | 1.33s | 5 | | 2. | 26.79s | Heat 2. | | 5. Tatiana Barbosa | --- | 56.47s | 1.65s | 4 | 0.79s | 3. | 27.27s | Heat 3. | | 6. Nadia Colovini | --- | 56.66s | 1.84s | 6 | 0.77s | 3. | 27.27s | Heat 2. | | 7. Arlene Semeco | --- | 56.69s | 1.87s | 3 | | 1. | 27.10s | Heat 3. | | 8. Liliana Ibanez | --- | 56.83s | 2.01s | 3 | 0.63s | 5. | 27.86s | Heat 1. | | 9. Daynara De Paula | --- | 57.14s | 2.32s | 5 | | 2. | 27.33s | Heat 1. | | 10.Caroline Lapierre | --- | 57.43s | 2.61s | 3 | | 4. | 27.35s | Heat 2. | | 11.Ariel Weech | --- | 57.97s | 3.15s | 2 | 0.77s | 4. | 27.85s | Heat 1. | | 12.Wendy Rodriguez | --- | 58.14s | 3.32s | 6 | 0.80s | 3. | 27.52s | Heat 1. | | 13.Karen Torrez | --- | 58.81s | 3.99s | 2 | | 5. | 28.02s | Heat 2. | | 14.Chinyere Pigot | --- | 58.95s | 4.13s | 6 | 0.80s | 4. | 27.96s | Heat 3. | | 15.Allyson Ponson | --- | 59.59s | 4.77s | 7 | | 6. | 28.32s | Heat 1. | | 16.Karen Nathalia Riveros | --- | 1:00.11 | 5.29s | 7 | 0.66s | 6. | 28.83s | Heat 3. | | 17.Julimar Avila | --- | 1:00.15 | 5.33s | 1 | | 5. | 28.63s | Heat 3. | | 18.Marie Meza Peraza | --- | 1:01.13 | 6.31s | 1 | | 6. | 28.79s | Heat 2. | | 19.Britany Van Lange | --- | 1:02.28 | 7.46s | 1 | | 7. | 29.10s | Heat 1. | | -. Fernanda Gonzalez | --- | DNS | | 2 | | | | Heat 3. | | -. Kendese Nangle | --- | DNS | | 7 | | | | Heat 2. |
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