| Final Thursday 06.08 18:49 | time | gap | L | React. | | 1. Yuanhui Fu |  CHN | 27.11s | | 4 | 0.65 | | 2. Etiene Medeiros |  BRA | 27.26s | AM0.15 | 5 | 0.64 | | 3. Xiang Liu | CHN | 27.58s | 0.47 | 6 | 0.63 | | 4. Emily Seebohm |  AUS | 27.66s | 0.55 | 2 | 0.60 | | 5. Mie Oe Nielsen |  DEN | 27.73s | 0.62 | 3 | 0.62 | | 6. Madison Wilson | AUS | 27.92s | 0.81 | 7 | 0.55 | | 7. Lauren Alice Quigley |  GBR | 27.99s | 0.88 | 1 | 0.61 | | 8. Theodora Drakou |  GRE | 28.17s | 1.06 | 8 | 0.58 |
| World and World Championship Record: Jing Zhao (CHN) 27.06s, Roma (ITA), 30.07.2009. | | World Junior Record: World Best Time. | | Qualification times. Standard A: 28.60s, Standard B: 29.60s |
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| Ranking | time | gap | L | React. | Semi | birth | | 1. Yuanhui Fu | CHN | 27.18s | | 4 | 0.61 | Semi 2 | 1996 | | 2. Etiene Medeiros | BRA | 27.41s | 0.23 | 4 | 0.60 | Semi 1 | 1991 | | 3. Mie Oe Nielsen | DEN | 27.63s | 0.45 | 3 | 0.66 | Semi 2 | 1996 | | 4. Xiang Liu | CHN | 27.67s | 0.49 | 5 | 0.63 | Semi 1 | 1996 | | 5. Emily Seebohm | AUS | 27.70s | 0.52 | 5 | 0.65 | Semi 2 | 1992 | | 6. Madison Wilson | AUS | 27.83s | 0.65 | 6 | 0.57 | Semi 1 | 1994 | | 7. Lauren Alice Quigley | GBR | 27.88s | 0.70 | 3 | 0.59 | Semi 1 | 1995 | | 8. Theodora Drakou | GRE | 28.01s | 0.83 | 7 | 0.55 | Semi 1 | 1992 | | eliminated | | 9. Anastasiia Fesikova |  RUS | 28.11s | 0.93 | 2 | 0.77 | Semi 1 | 1990 | | 10.Mathilde Cini |  FRA | 28.16s | 0.98 | 8 | 0.66 | Semi 2 | 1994 | | 11.Elena Gemo |  ITA | 28.21s | 1.03 | 7 | 0.71 | Semi 2 | 1987 | | 12.Kirsty Leigh Coventry |  ZIM | 28.28s | 1.10 | 2 | 0.62 | Semi 2 | 1983 | | 13.Beryl E. Gastaldello | FRA | 28.30s | 1.12 | 6 | 0.57 | Semi 2 | 1995 | | 14.Daria K Ustinova | RUS | 28.39s | 1.21 | 8 | 0.62 | Semi 1 | 1998 | | 15.Aleksandra Urbanczyk |  POL | 28.46s | 1.28 | 1 | 0.66 | Semi 2 | 1987 | | 16.Sanja Jovanovic |  CRO | 28.59s | 1.41 | 1 | 0.67 | Semi 1 | 1986 |
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| Ranking | time | gap | L | React. | Heat | birth | | 1. Yuanhui Fu | CHN | 27.66s | | 4 | 0.69 | Heat 4 | 1996 | | 2. Etiene Medeiros | BRA | 27.74s | 0.08 | 4 | 0.65 | Heat 6 | 1991 | | 3. Emily Seebohm | AUS | 27.75s | 0.09 | 4 | 0.61 | Heat 5 | 1992 | | 4. Xiang Liu | CHN | 27.79s | 0.13 | 5 | 0.64 | Heat 5 | 1996 | | 5. Mie Oe Nielsen | DEN | 27.89s | 0.23 | 5 | 0.66 | Heat 4 | 1996 | | 6. Lauren Alice Quigley | GBR | 27.94s | 0.28 | 5 | 0.61 | Heat 6 | 1995 | | 7. Beryl E. Gastaldello | FRA | 28.02s | 0.36 | 6 | 0.58 | Heat 4 | 1995 | | 7. Madison Wilson | AUS | 28.02s | 0.36 | 3 | 0.62 | Heat 6 | 1994 | | 9. Kirsty Leigh Coventry | ZIM | 28.15s | 0.49 | 6 | 0.71 | Heat 5 | 1983 | | 9. Anastasiia Fesikova | RUS | 28.15s | 0.49 | 2 | 0.69 | Heat 6 | 1990 | | 11.Elena Gemo | ITA | 28.18s | 0.52 | 6 | 0.70 | Heat 6 | 1987 | | 12.Theodora Drakou | GRE | 28.35s | 0.69 | 0 | 0.57 | Heat 6 | 1992 | | 13.Aleksandra Urbanczyk | POL | 28.36s | 0.70 | 7 | 0.61 | Heat 4 | 1987 | | 14.Sanja Jovanovic | CRO | 28.40s | 0.74 | 8 | 0.67 | Heat 6 | 1986 | | 15.Mathilde Cini | FRA | 28.46s | 0.80 | 1 | 0.70 | Heat 6 | 1994 | | 16.Daria K Ustinova | RUS | 28.52s | 0.86 | 2 | 0.62 | Heat 5 | 1998 | | eliminated | | 17.Sviatlana Khakhlova |  BLR | 28.62s | 0.96 | 8 | 0.74 | Heat 5 | 1984 | | 18.Katarina Listopadova |  SVK | 28.63s | 0.97 | 0 | 0.65 | Heat 5 | 1993 | | 19.Dominique Bouchard |  CAN | 28.66s | 1.00 | 9 | 0.60 | Heat 5 | 1991 | | 20.Yekaterina Rudenko |  KAZ | 28.67s | 1.01 | 3 | 0.76 | Heat 5 | 1994 | | 20.Simona Baumrtova |  CZE | 28.67s | 1.01 | 1 | 0.63 | Heat 5 | 1991 | | 22.Hoi Shun Stephanie Au |  HKG | 28.70s | 1.04 | 9 | 0.59 | Heat 6 | 1992 | | 23.Eyglo Osk Gustafsdottir |  ISL | 28.75s | 1.09 | 0 | 0.74 | Heat 4 | 1995 | | 24.Hanbyeol Park |  KOR | 28.85s | 1.19 | 7 | 0.57 | Heat 5 | 1997 | | 25.Maaike De Waard |  NED | 28.86s | 1.20 | 7 | 0.70 | Heat 6 | 1996 | | 26.Alicja Tchorz | POL | 28.87s | 1.21 | 1 | 0.63 | Heat 4 | 1992 | | 27.Gisela Morales |  GUA | 28.94s | 1.28 | 5 | 0.73 | Heat 3 | 1987 | | 28.Daryna Zevina |  UKR | 28.97s | 1.31 | 8 | 0.72 | Heat 4 | 1994 | | 29.Bobbie Dene Gichard |  NZL | 29.15s | 1.49 | 9 | 0.57 | Heat 4 | 1999 | | 30.Magdalena Kuras |  SWE | 29.33s | 1.67 | 4 | 0.67 | Heat 3 | 1988 | | 31.Naomi Ruele |  BOT | 29.36s | 1.70 | 5 | 0.78 | Heat 2 | 1997 | | 32.Yulduz Kuchkarova |  UZB | 29.37s | 1.71 | 6 | 0.69 | Heat 3 | 1994 | | 33.Amit Ivry |  ISR | 29.50s | 1.84 | 3 | 0.61 | Heat 3 | 1989 | | 34.Ekaterina I. Avramova |  TUR | 29.60s | 1.94 | 2 | 0.55 | Heat 3 | 1991 | | 35.Carolina Colorado Henao |  COL | 29.61s | 1.95 | 0 | 0.68 | Heat 3 | 1987 | | 36.Sigrid Sepp |  EST | 29.95s | 2.29 | 7 | 0.65 | Heat 3 | 1998 | | 37.Caroline Zi Xin Chan |  MAS | 30.14s | 2.48 | 9 | 0.65 | Heat 3 | 1995 | | 38.Jessika Lyudmila Cossa |  MOZ | 30.17s | 2.51 | 4 | 0.71 | Heat 2 | 1995 | | 39.Anak Agung Istri Kania Ratih |  INA | 30.33s | 2.67 | 1 | 0.55 | Heat 3 | 1998 | | 40.Ariel Weech |  BAH | 30.77s | 3.11 | 3 | 0.68 | Heat 2 | 1991 | | 41.Monica Ramirez Abella |  AND | 30.89s | 3.23 | 2 | 0.61 | Heat 2 | 1993 | | 42.Andrea Garcia |  MEX | 30.94s | 3.28 | 8 | 0.63 | Heat 3 | 1992 | | 43.Evelina Afoa |  SAM | 31.74s | 4.08 | 7 | 0.66 | Heat 2 | 1998 | | 44.Lauren HEW |  CAY | 32.33s | 4.67 | 6 | 0.55 | Heat 2 | 1999 | | 45.Colleen Furgeson |  MHL | 33.04s | 5.38 | 1 | 0.77 | Heat 2 | 1998 | | 46.Rita Zeqiri |  KOS | 33.65s | 5.99 | 0 | 0.68 | Heat 2 | 1995 | | 47.Rahaf Jamal Sa'D Baqleh |  JOR | 33.67s | 6.01 | 8 | 0.65 | Heat 2 | 1998 | | 48.Debra Daniel |  FSM | 33.75s | 6.09 | 9 | 0.64 | Heat 2 | 1991 | | 49.Yesui Bayar |  MGL | 35.44s | 7.78 | 4 | 0.75 | Heat 1 | 2000 | | 50.Anastasiya Tyurina |  TJK | 38.48s | 10.82 | 5 | | Heat 1 | 2001 | | 51.Tayamika Chang'anamuno |  MAW | 38.81s | 11.15 | 3 | 0.72 | Heat 1 | 2000 | | 52.A.Abdulmohsen Ahmed |  BRN | 40.40s | 12.74 | 6 | 0.87 | Heat 1 | 1999 | | -. Mimosa Jallow |  FIN | DSQ | | 2 | | Heat 4 | 1994 | | -. Rachel Bootsma |  USA | DNS | | 3 | | Heat 4 | 1993 |
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| Swimmer | Q. time | Birth | L | Heat | | 2. Etiene Medeiros | BRA | 27.38s | 1991 | 4 | Heat 6 | | 4. Emily Seebohm | AUS | 27.47s | 1992 | 4 | Heat 5 | | 1. Yuanhui Fu | CHN | 27.61s | 1996 | 4 | Heat 4 | | 7. Lauren Alice Quigley | GBR | 27.69s | 1995 | 5 | Heat 6 | | 3. Xiang Liu | CHN | 27.73s | 1996 | 5 | Heat 5 | | 5. Mie Oe Nielsen | DEN | 27.76s | 1996 | 5 | Heat 4 | | 6. Madison Wilson | AUS | 27.88s | 1994 | 3 | Heat 6 | | -. Yekaterina Rudenko | KAZ | 28.04s | 1994 | 3 | Heat 5 | | -. Rachel Bootsma | USA | 28.05s | 1993 | 3 | Heat 4 | | -. Elena Gemo | ITA | 28.07s | 1987 | 6 | Heat 6 | | -. Kirsty Leigh Coventry | ZIM | 28.08s | 1983 | 6 | Heat 5 | | -. Beryl Emilie Paulette Gastaldello | FRA | 28.10s | 1995 | 6 | Heat 4 | | -. Anastasiia Fesikova | RUS | 28.18s | 1990 | 2 | Heat 6 | | -. Daria K Ustinova | RUS | 28.18s | 1998 | 2 | Heat 5 | | -. Mimosa Jallow | FIN | 28.20s | 1994 | 2 | Heat 4 | | -. Maaike De Waard | NED | 28.29s | 1996 | 7 | Heat 6 | | -. Hanbyeol Park | KOR | 28.32s | 1997 | 7 | Heat 5 | | -. Aleksandra Urbanczyk | POL | 28.38s | 1987 | 7 | Heat 4 | | -. Mathilde Cini | FRA | 28.42s | 1994 | 1 | Heat 6 | | -. Simona Baumrtova | CZE | 28.46s | 1991 | 1 | Heat 5 | | -. Alicja Tchorz | POL | 28.48s | 1992 | 1 | Heat 4 | | -. Sanja Jovanovic | CRO | 28.50s | 1986 | 8 | Heat 6 | | -. Sviatlana Khakhlova | BLR | 28.58s | 1984 | 8 | Heat 5 | | -. Daryna Zevina | UKR | 28.59s | 1994 | 8 | Heat 4 | | Standard A: 28.60s | | 8. Theodora Drakou | GRE | 28.61s | 1992 | 0 | Heat 6 | | -. Katarina Listopadova | SVK | 28.61s | 1993 | 0 | Heat 5 | | -. Eyglo Osk Gustafsdottir | ISL | 28.63s | 1995 | 0 | Heat 4 | | -. Hoi Shun Stephanie Au | HKG | 28.67s | 1992 | 9 | Heat 6 | | -. Dominique Bouchard | CAN | 28.94s | 1991 | 9 | Heat 5 | | -. Bobbie Dene Gichard | NZL | 28.99s | 1999 | 9 | Heat 4 | | -. Magdalena Kuras | SWE | 29.10s | 1988 | 4 | Heat 3 | | -. Gisela Morales | GUA | 29.14s | 1987 | 5 | Heat 3 | | -. Amit Ivry | ISR | 29.25s | 1989 | 3 | Heat 3 | | -. Yulduz Kuchkarova | UZB | 29.31s | 1994 | 6 | Heat 3 | | -. Ekaterina Ivanova Avramova | TUR | 29.34s | 1991 | 2 | Heat 3 | | -. Sigrid Sepp | EST | 29.42s | 1998 | 7 | Heat 3 | | -. Anak Agung Istri Kania Ratih | INA | 29.48s | 1998 | 1 | Heat 3 | | -. Andrea Garcia | MEX | 29.57s | 1992 | 8 | Heat 3 | | -. Carolina Colorado Henao | COL | 29.58s | 1987 | 0 | Heat 3 | | -. Caroline Zi Xin Chan | MAS | 29.60s | 1995 | 9 | Heat 3 | | Standard B: 29.60s | | -. Jessika Lyudmila Cossa | MOZ | 30.08s | 1995 | 4 | Heat 2 | | -. Naomi Ruele | BOT | 30.43s | 1997 | 5 | Heat 2 | | -. Ariel Weech | BAH | 30.54s | 1991 | 3 | Heat 2 | | -. Lauren Hew | CAY | 30.55s | 1999 | 6 | Heat 2 | | -. Monica Ramirez Abella | AND | 31.01s | 1993 | 2 | Heat 2 | | -. Evelina Afoa | SAM | 32.28s | 1998 | 7 | Heat 2 | | -. Colleen Furgeson | MHL | 33.19s | 1998 | 1 | Heat 2 | | -. Rahaf Jamal Sa'D Baqleh | JOR | 33.39s | 1998 | 8 | Heat 2 | | -. Rita Zeqiri | KOS | 33.60s | 1995 | 0 | Heat 2 | | -. Debra Daniel | FSM | 34.30s | 1991 | 9 | Heat 2 | | -. Yesui Bayar | MGL | 35.95s | 2000 | 4 | Heat 1 | | -. Anastasiya Tyurina | TJK | 36.00s | 2001 | 5 | Heat 1 | | -. Tayamika Chang'anamuno | MAW | 39.90s | 2000 | 3 | Heat 1 | | -. F. Abdulmohsen Ahmed Almahme | BRN | 42.13s | 1999 | 6 | Heat 1 |
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| Theodora Drakou (GRE) with Standard B qualified to the Final. |
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